Field application of two strains of Nomuraea rileyi (Farlow) against larvae of Spodoptera frugiperda (J. E. Smith) in corn, in Pesquería, Nuevo León, Mexico
DOI:
https://doi.org/10.59741/agraria.v14i1.532Keywords:
Entomopathogenic, Nomuraea rileyi, Spodoptera frugiperdaAbstract
Zea mays L. ssp mays, is distributed throughout the Mexican territory (Mera and Mapes, 2009), and is known as cultivated maize, corn is one of the most important crops in the world and agricultural species more diverse in Mexico. Entomopathogenic fungi, play an important role in biological pest control, due to its mode of action, ease of application and the ability to generate animal diseases under field conditions (Ignoffo, 1981; Alves, 1986). Laboratory tests and field tests were conducted. Different culture media for isolation of the fungus tested. Subsequently obtaining both pure and isolates the first procedure consisted of inoculating larvae 3rd instar obtained from a breeding stock laboratory Inoculation was performed as topical application, with a liquid at a concentration of 107 suspension were placed 200 grams of rice per bag, was sterilized in an autoclave at 121° C (15 pounds pressure) for 15 minutes in plastic bags, rice was inoculated with 15 ml of conidial suspension and allowed to incubate at 25° C for two weeks. a design randomized block with five treatments and four replications, each experimental unit consisted of four rows. For applications of treatments, 20 linear consecutive plants were considered furrow In phase laboratory test was established, they were compared means pda, pda + antibiotic, egg-agar, soy-agar, obtaining as best result means agar-soybean, as was the only sporulation occurred while on PDA were only mycelial growth in the remaining half no growth. In the field results in the germination tests strains, prior to the application we obtained for n = 400 conidia was obtained for NH03CT = 84% and C3 = 70% of germination. In all treatment there were highly significant differences variables for mortality and damage fungi infested plant, concluding that the strains used are a great alternative for handling Spodoptera frugiperda.
Downloads
References
Andrews K. L., 1988. Latin american research on Spodoptera frugiperda (Lepidoptera: Noctuidae). Florida Entomologist 71, 630-653. DOI: https://doi.org/10.2307/3495022
Boucias, D. G., Schoborg, E. A., and Allen, G. E. 1982. The relative susceptibility of six noctuid species to in- fection by Nomuraea rileyi isolated from Anticarsia gemmatalis. J. Invertebr. Pathol. 39, 238-240. DOI: https://doi.org/10.1016/0022-2011(82)90017-9
Boucias, D., Stokes, C., Suazo, A. and Funderburk, J.2000. AFLP Analysis of Nomuraea rileyi DNA. Mycologia, in press.
Bustillo, A., Posada, F. 1986. Patogenicidad de un aislamiento de Nomuraea rileyi sobre larvas del cogollero del maíz Spodopíera frugiperda. Revista Colombiana de Entomología 12 (1): 5-15. DOI: https://doi.org/10.25100/socolen.v12i1.10223
Casmuz, A. M., L. Juárez, M. G. Socías, M. G. Murúa, S.Prieto, S. M, E. Willink y G. Gastaminza. Revisión de los hospederos del gusano cogollero del maíz, Spodoptera frugiperda (Lepidoptera: Noctuidae). Rev. Soc. Eedinantomol. Argent. 2010, 69 (3-4), 209-231.
Correa, B. S., and Smith, J. G. 1975. Nomuraea rileyi attacking the velvetbean caterpillar, Anticarsia gemmatalis Hubner in Parana ́. Fla. Entomol. 58, 280. DOI: https://doi.org/10.2307/3493691
Ferraz, J. M. G., Patel, P. M., and Habib, M. E. M. 1991. Cross infection of Nomuraea rileyi isolated from Spodoptera frugiperda and Anticarsia gemmatalis. In “Abstracts of the 12th International Plant Protection Congress at Rio de Janeiro”.
Herrera, A. J. Principales plagas del maíz. Boletín Especial de la Dirección de Agricultura y Ganadería del Perú. 1979.
Ignoffo, C. M. 1981. The fungus Nomuraea rileyi as a microbial insecticide, In “Microbial Control of Pests and Plant Diseases: 1970-1980” (H. D. Burges, Ed.), pp. 513-538. Academic Press, London.
Ignoffo, C. M., and Boucias, D. B. 1992. Relative activity of geographical isolates of Nomuraea bioassayed against the cabbage looper and velvetbean caterpillar. J. Invertebr. Pathol. 59, 215-217. DOI: https://doi.org/10.1016/0022-2011(92)90039-7
Ignoffo, C. M., Puttler, B., Hostetter, D. L., and Dickerson, W. A. 1976. Susceptibility of the cabbage looper, Trichoplus Ia ni, and the velvetbean caterpillar, Anticarsia gemmatalis, to several isolates of the entomopathogenic fungus nomuraea rileyi. J. Invertebr. Pathol. 28, 259-262. DOI: https://doi.org/10.1016/0022-2011(76)90132-4
Joslyn, D. J., and Boucias, D. G. 1981. Isozyme differentiation among three pathotypes of the entomogenous fungus Nomuraea rileyi. Can. J. Microbiol. 27, 364-366. DOI: https://doi.org/10.1139/m81-055
Kato, T. A., C. Mapes, L. M. Mera, J. A. Serratos y R. A. Bye. Origen y diversificación del maíz: una revisión analítica. Universidad Nacional Autónoma de México, Comisión Nacional para el Conocimiento y Uso de la Biodiversidad. México, D.F. 2009. Pág.116.
Kish, L. P., and Allen, G. E. 1978. The biology and ecology of Nomuraea rileyi and a program for predicting its incidence on Anticarsia gemmatalis in soybean. Univ. Fla. Agric. Exp. Station. Tech. Bull. 795, 1-58.
Kish, L. P., Samson, R. A., and Allen, G. E. 1974. The genus Nomuraea Maublanc. J. Invertebr. Pathol. 24, 154-158. DOI: https://doi.org/10.1016/0022-2011(74)90004-4
Mera, O. L. M. y S. C. Mapes. El Maíz. Aspectos Biológicos. En: Kato, T. A., C. Mapes, L. M. Mera, J. A. Serratos y R. A. Bye. Origen y diversificación del maíz: una revisión analítica. Universidad Nacional Autónoma de México, Comisión Nacional para el Conocimiento y Uso de la Biodiversidad. México, D.F. 2009. Pág. 116.
Morrow, B. J., Boucias, D. G., and Heath, M. A. 1989. Loss of virulence in an isolate of an entomopathogenic fungus, Nomuraea rileyi, after serial in vitro passage. J. Econ. Entomol. 82, 404-497 DOI: https://doi.org/10.1093/jee/82.2.404
Ortega, A. C. Insectos nocivos del maíz: una guía para su identificación en el campo. Centro Internacional de Mejoramiento de Maíz y Trigo (cimmyt). México. 1987, p. 106.
Pavone, D., and Dorta, B., 2010. Efecto de agroquímicos sobre el desarrollo del hongo entomopatogeno nomuraea rileyi y su virulencia sobre spodoptera frugiperda. Bioagro 22 (2).
Pendland, J. C., and Boucias, D. G. 1997. In vitro growth of the entomopathogenic hyphomycete Nomuraea rileyi. Mycologia 89, 66-71. DOI: https://doi.org/10.1080/00275514.1997.12026755
Rodríguez, D. L. A y A. J. Marín. Insectos plaga y su control. pp. 29-46. En: Rodríguez M. R. y C. De León. El cultivo del maíz. Temas selectos 1. Colegio de Postgraduados. Mundi Prensa México, 2008. pp. 127.
Sena D. G. Jr., F. A. C. Pinto, D. M. Queiroz and P. A. Viana. Fall Armyworm Damaged Maize Plant Identification using Digital Images. Biosystems Eng. 2003, 85(4), 449-454. DOI: https://doi.org/10.1016/S1537-5110(03)00098-9
Sifuentes, J. A. Plagas del maíz en México. INIFAP. Folleto técnico. Núm. 85. México D. F. 1985. Pág. 49.
Silva-Aguayo, G., J. C. Rodríguez-Maciel, A. Lagunes Tejeda, C. Landeral-Cázares, R. Alatorre-Rosas, A. M. Shelton and C. A. Blanco. Bioactivity of Boldo (Peumusboldus Molina) (Laurales: Monimiaceae) on Spodoptera frugiperda (J. E. Smith) and Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae). Southwest. Entomol.2010, 35(3), 215-231. DOI: https://doi.org/10.3958/059.035.0301
Tigano-Milani, M. S., Faria, M. R., Lecuona, R. E., Sartori, M. R., Arima, E. Y., and Diaz, B. M. 1995b. Ana ́lise de patogenicidade e germinac ̧a ̃o do fungo Nomuraea rileyi (Farlow) Samson isolado no Distrito Federal. Ann. Soc. Entomol. Brasil. 24, 53-60 DOI: https://doi.org/10.37486/0301-8059.v24i1.994
Tigano, M. S., and Aljanbi, S. 1999. RAPD analysis of Nomuraea rileyi. J. Invertebr. Pathol. 75, 240-242. DOI: https://doi.org/10.1006/jipa.1999.4920
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
How to Cite
PLUMX Metrics
