Nematophagus fungi Arthrobotrys spp., against Meloidogyne incognita under in vitro conditions
DOI:
https://doi.org/10.59741/agraria.v14i1.529Keywords:
Plant parasitic nematodes, biologic control, OrbiliaceaeAbstract
The root-knot nematode Meloidogyne incognita, is reported as the main nematode that causes severe damage to agriculture around the world. Nematophagous fungi such as Arthrobotrys spp are natural enemies of nematodes, which can immobilize and digest. In order to reduce the use of chemical nematicides, it aims to generate new technologies for biological control of Meloidogyne incognita, from the use of control agents such as fungi of the genus Arthrobotrys spp. The aim of this study was to identify species of the fungus genus Arthrobotrys with nematophagous activity against Meloidogyne incognita, in different agricultural regions of Mexico. This research was conducted during 2014. 150 samples of agricultural soils from a depth of 0-15 cm based on its own to obtain fungi and nematodes were processed methodologies. The fungi were identified using microscopy techniques and taxonomic keys, finding five different strains of nematophagous fungi, Arthrobotrys musiformis (HN01), Drechslerella yunnanensis (HN02), Arthrobotrys oligospora (HN03), Artrobotrys spp., (HN04), Artrobotrys musiformis. (HN05). Nematophagous bioassays activity of each identified species of the genus Arthobotrys against Meloidogyne incognita under in vitro conditions were performed. These were conducted in Petri dish 5 cm diameter, containing second stage juveniles of Meloidogyne incognita, adding concentrated cell 1x105, 1x106 and 1x107 for a period of 168h. A completely random design was established factorial arrangement with 15 treatments with 5 more reps an additional witness, the data obtained were analyzed by R. The percent mortality was evaluated by performing an analysis of variance between treatments and TL50 average by probit analysis. The nematophagous capacity was 94% HN01 TL50 106.07h; 146.53h TL50 59% HN02; 118.24h TL50 87% HN03; 136.98h TL50 69% HN04; 131.88h TL50 72% HN05.
Downloads
References
Barker, K. R., & Koenning, S. R. (1998). Developing sustainable systems for nematode management.Annua lReview of Phytopathology, 36, 165-205. DOI: https://doi.org/10.1146/annurev.phyto.36.1.165
Carpenter, J., Lynch, L., & Trout, T. (2001). Township limits on 1,3-D will impact adjustment to methyl bromide phase-out. California Agriculture, 55, 12-18. DOI: https://doi.org/10.3733/ca.v055n03p12
Chitwood, D. J. (2003). Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture-Agricultural Research Service. Pest Management Science, 59, 748-753. DOI: https://doi.org/10.1002/ps.684
Dávila, L. (2005). Evaluación de la actividad biocontroladora de Arthrobotrys sp. y Paecilomyces sp. Sobre Meloidogyne javanica in vitro y bajo condiciones de invernadero en crisantemo (Drendranthema grandiflora Andernson). Agronomía colombiana.
Eilenberg, J., Hajek, A., & Lomer, C. (2001). Suggestions for unifying the terminology in biological control. BioControl, 46, 387-400. DOI: https://doi.org/10.1023/A:1014193329979
Gams, W., & Zare, R. (2003). A taxonomic review of the clavicipitaceous anamorphs parasitizing nematodes and other microinvertebrates. In J. F. White Jr., C. W. Bacon, N. L. DOI: https://doi.org/10.1201/9780203912706.pt1
Gutiérrez, A. & Mendoza, P. (2013). Hongos nematófagos (Orbiliales) capturando, destruyendo y alimentándose de larvas hisotrópicas de Haemonchus contortus (Nematoda: Trichostrongylidae). Revista Mexicana de Micología.
Hugot, J. P., Baujard, P., & Morand, S. (2001). Biodiversity in helminths and nematodes as a field of study: Anoverview. Nematology, 3, 199-208. DOI: https://doi.org/10.1163/156854101750413270
Hywel-Jones & J. W. Spatafora (Eds.), Clavicipitalean fungi: Evolutionary biology, chemistry, and cultural impacts. New York: Marcel Dekker.
Jaffee, B., & Strong, D. (2005). Strong bottom-up and weak top-down effects in soil: Nematodeparasitized insects and nematode-trapping fungi. Soil Biology and Biochemistry, 37, 1011-1021. DOI: https://doi.org/10.1016/j.soilbio.2004.05.026
Mitsui, Y. (1985). Distribution and ecology of nematode trapping fungi in Japan. JARQ, 18, 182-193.
Molinari, S. (2011). Natural genetic and induced plant resistance, as a control strategy to plantparasitic nematodes alternative to pesticides. Plant Cell Reports, 30, 311–323. DOI: https://doi.org/10.1007/s00299-010-0972-z
Nordbring-Hertz, B., Jansson, H. B., & Tunlid, A. (2006). Nematophagous fungi. In Encyclopedia of life sciences. Chichester: John Wiley & Sons. DOI: https://doi.org/10.1038/npg.els.0004293
Park, J., Gams, W., Scholler, M., Ghisalberti, E., & Sivasithamparam, K. (2002). Orbiliaceous nematode-trapping fungi and related species in Western Australia and their biological activities. Australasian Mycologist, 21, 45-52.
Persmark, L., & Jansson, H. B. (1997). Nematophagous fungi in the rhizosphere of agricultural crops. FEMS Microbiology Ecology, 22, 303-312. DOI: https://doi.org/10.1016/S0168-6496(97)00006-8
Sasser, J. N. (1980). Root-knot nematodes: A global menace to crop production. Plant Disease, 64, 36-41. DOI: https://doi.org/10.1094/PD-64-36
Tian, B. Y., Yang, J. K., & Zhang, K. Q. (2007). Bacteria used in the biological control of plantparasitic nematodes: Populations, mechanisms of action, and future prospects. FEMS Microbiology Ecology, 61, 197-213. DOI: https://doi.org/10.1111/j.1574-6941.2007.00349.x
Zhang, Y., Li, G. H., & Zhang, K. Q. (2011). A review on the research of nematophagous fungal species (In Chinese). Mycosystema, 30, 836-845.
Zopf, W. (1888). Zur Kenntnis der Infektionskrankheiten niederer Thiere und Pflanzen. Nova Acadamy of Caes.Leop. German. Nat. Cur, 52, 314-376.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
How to Cite
PLUMX Metrics
