Relación entre fracciones de proteínas con la germinación, vigor y sanidad en semilla de maíz
DOI:
https://doi.org/10.59741/agraria.v2i2.327Keywords:
Genotype, Fusarium moniliforme, albumen, globulin, zein, glutelinAbstract
Relation among protein fractions with germination, vigor and health in maize seed. The concentrations of zein, glutelin, albumen and globulin in whole seed, embryo and endosperm of nine maize genotypes were determined, with the purpose of studying the relations between the types, and the concentrations of proteins, with germination, vigor and health of the seed, before and after putting them under artificial aging. A completely randomized design with four repetitions was applied. The fractions of proteins were quantified by Lowry’s method of (1951). The relation between variables was studied by means of the analysis of main components. The results showed that all the genotypes, but G4, presented an infection by Fusarium moniliforme, which was positively related to the concentration of albumen, globulin and zein in whole seed, as well as with the abnormal appearance of plantules. Germination and vigor were positively related to the concentration of glutelin in the embryo, endosperm and whole grain. The vigor, determined by means of length of plumule and dry weight, was positively related to the concentrations of glutelin in endospero and zein in the embryo. The conclusion is that the presence of F. moniliforme in the seed, in most of the evaluated genotypes, affected its physiological quality, and the proliferation of the pathogen was positively related to the concentrations of albumen and globulin. Genotypes G1 and G3 showed the highest values of infection by F. moniliforme, which caused both a loss in germination and in vigor. The greater similarity in the evaluated characteristics was the one between the Genotypes G7 and G9 with relation to the G2, used like witness.
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AOSA (Association of Official Seed Analysts). 1992. Seedling evaluation handbook. Contribution No. 35. The Handbook of official Seed. USA. pp: 76- 80
AOSA (Association of Official Seed Analysts). 1983. Seed vigor testing handbook. Contribution No. 32. The Handbook of official Seed. USA. 88 p.
Bin Mohammad K. and Esen A. 1990. Zein degradation in the endosperm of maize seeds during germination. Amer. J. Bot. 77 (8): 973-980.
Booth C. 1971. The genus Fusarium. Commonwealth Mycological Institute (Ed.). Kew, Surrey England. 237
Boyer R. 2000. Conceptos en Bioquímica. International Thomson (Eds.), D.F. México pp: 92-97, 257-265. Carrillo R. J. 2003. Micotoxinas. El veneno más potente
de la naturaleza. Revista on Line Tattersall. (Ed.). 181 mayo-junio.
Carvalho, N.M.; Nakagawa, J. 1988. Sementes: ciência, tecnologia e produção. 3 Ed. Campinas: Fundação Cragill. 424 p.
De Barros E. G. y Larkins B.A. 1990. Purification and characterization of zein-degrading proteases from endosperm of germinating maize seeds. Plant Physiol. 94: 297-303 DOI: https://doi.org/10.1104/pp.94.1.297
Dunwell JM, Khuri S, Gane PJ. 2000. Microbial relatives of the seed storage proteins of higher plants: conservation of structure and diversification of function during evolution of the cupin superfamily. Microbiology and Molecular Biology Reviews 64:153-179. DOI: https://doi.org/10.1128/MMBR.64.1.153-179.2000
FAO. 2003. http://faostat.fao.org/default.jsp/language=ES. Forato L. A., L.A. Colnago, R.C. Garratt and M.A. Lopes. 2000. Identification of free fatty acids in maize protein bodies and purified ±-zeins by 13C and 1H nuclear magnetic resonance. Biochimica et Biophysica Acta (BBA)/ Protein Structure and Molecular Enzymology. 1543 (1): 106-114. DOI: https://doi.org/10.1016/S0167-4838(00)00190-4
Garay, A. E. 1989. La calidad de la semilla y sus componentes. pp: 2-11. In: Memorias del primer curso avanzado sobre sistemas de semillas para pequeños agricultores. Centro Internacional de Agricultura Tropi cal (CIAT). Cali, Colombia.
Guo, B. Z., Chen, Z.-Y., Brown, R. L., Lax, A. R., Cleve land, T. E., Russin, J. S., Mehta, A. D., Selitrennikoff, C. P., and Widstrom, N. W. 1997. Germination induces accumulation of specific proteins and antifungal activities in corn kernels. Phytopathology 87:1174-1178. DOI: https://doi.org/10.1094/PHYTO.1997.87.11.1174
Hampton, J.G. 2001. ¿Qué significa calidad de semillas? Seed News. Septiembre/octubre, 5 (5): 1-2
Hashem, M.; Zohri, A. A. 2003. Morphological, phytophahological, toxicological, enzymological characteristics and molecular variation of some strains of Fusarium graminearum and F. equiseti. African Journal of Mycology and Biotechnology 11 (1): 1-20.
Hojilla- Evangelista, P. Mila, Johnson and A. Lawrence. 2003. Optimizing extraction of zein and glutelin-rich fraction during sequential extraction processing of corn. Cereal Chemistry 80 (4): 481-484. DOI: https://doi.org/10.1094/CCHEM.2003.80.4.481
Johnson, R.A. and D.W. Wichern. 1988. Applied multi variate statistical analysis. 2nd edition. Editorial Prentice Hall. New Jersey. USA. 607 p.
ISTA (International Seed Testing Association). 1996.
Internacional Rules for Seed Testing. Rules 1996. Seed Sci. Technol. 13 (2): 299-355.
Larsen, A. 1967. Electrophoretic differences in seed proteins among varieties of soybean, Glycine max (L.) Merrill. Crop. Sci. 7: 311-313. DOI: https://doi.org/10.2135/cropsci1967.0011183X000700040008x
Lowry, O.H., Rosebrough N.J., Farr A.L. and Randall R.J. 1951. Protein measurement with the Folin phenol re agent. J. Biol Chem.193: 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Manly, B.F.J. 1986. Multivariate statistical methods: First edition Chapman and
Hall (Eds.). London. 160p.
Monjardino P., A.G. Smith and R. J. Jones. 2005. Heat stress effects on protein accumulation of maize endosperm. Crop Sci. 45 (4): 1203-1211. DOI: https://doi.org/10.2135/cropsci2003.0122
Neergaard, P. 1977. Seed Pathology, Vol. I and Vol. II. John Wiley & Sons, New York. DOI: https://doi.org/10.1007/978-1-349-02842-9
Ortiz, L. 1992. Extracción y caracterización de las prolaminas del grano de seis cultivares de sorgo (Sor ghum bicolor (L.) Moench. Arch. Latinoamer. Nutr. 42 (1): 46-51.
Parra, P. 1990. Caracterización de híbridos venezolanos de sorgo (Sorghum bicolor (L.) Moench), Prosevenca 5, Criollo 1, Chaguaramas 3 y sus parentales, mediante la aplicación de electroforesis discontinua en gel de poliacrilamida y sus estudios morfológicos. Tesis de maestría. Facultad de Agronomía, Maracay Universidad Central. de Venezuela. 89 p.
Parra, P. y Ortiz L. 1993. Montaje de un método electroforético para la identificación de cultivares de maíz. Rev. Fac. Agron. Maracay. Universidad Central de Venezuela. 19 (3): 213-226.
Perry, D. A. 1977. A vigor test for seeds of barley (Hordeum vulgare) based on measurement of plumule growth. Seed Science and Technology 5: 709-719.
Perry, D. A. 1987. Introduction: methodology and application of vigourtests: Growth and evaluation tests: To pographical Tetrazolium test. International Seed Testing Association (ISTA). Handbook of vigourtests methods. 2nd edition, Zurich, Switzerland. p.72
SAS Institute Inc. 1989. SAS/STAT User’s guide. Version 6. Fourth Edition. SAS Institute. Cary, NC. USA.
Sato, K., Yamagishi T. and Yamauchi F. 1986. Quantitative analisys of soybean proteins by densitometry on gel electrophoresis. Cereal Chem. 63 (6): 493-496.
SNICS (Servicio Nacional de Inspección y Certificación de Semillas). 1980. Normas para la certificación de semillas. Secretaria de Agricultura y Ganadería, Dirección General de Agricultura. México. pp: 66-67.
Shutov A.D., H. Bäumlein, F.R. Blattner and K. Müntz.
Storage and mobilization as antagonistic functional constraints on seed storage globulin evolution. Journal of Experimental Botany 54 (388): 1645-1654. DOI: https://doi.org/10.1093/jxb/erg165
Steel, G.D. and J.H. Torrie. 1980. Principles and procedures of statistics. McGraw-Hill (Ed.), New York. USA. Thomson, J. R. 1979. An introduction to seed technology.
Thomson Litho Ltd. (Ed.), Great Britain. pp: 1-15. Association of Official Seed Analysts (AOSA) 1992. Seed ling evaluation handbook. Contribution No. 35 The Handbook of official Seed. United Status of America. 76- 80 p.
Association of Official Seed Analysts (AOSA) 1983. Seed vigor testing handbook. Contribution No. 32 The Handbook of official Seed. United Stateus of America. 88p.
Bin Mohammad K. and Esen A. 1990. Zein degradation in the endosperm of maize seeds during germination. Amer. J. Bot. 77(8): 973-980. DOI: https://doi.org/10.1002/j.1537-2197.1990.tb13591.x
Booth C. 1971. The genus Fusarium kew surrey England. Commonwealth Micological Institute. 237 p. Boyer R. 2000. Conceptos en Bioquímica. International Thomson Editores, S.A. de C.V., México pp. 92-97, 257-265.
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